Gibberellin-Induced Changes in the Populations of Translatable mRNAs and Accumulated Polypeptides in Dwarfs of Maize and Pea.

نویسندگان

  • J Chory
  • D F Voytas
  • N E Olszewski
  • F M Ausubel
چکیده

Two-dimensional gel electrophoresis was used to characterize the molecular mechanism of gibberellin-induced stem elongation in maize and pea. Dwarf mutants of maize (d-5) and pea (Progress No. 9) lack endogenous gibberellin (GA(1)) but become phenotypically normal with exogenous applications of this hormone. Sections from either etiolated maize or green pea seedlings were incubated in the presence of [(35)S] methionine for 3 hours with or without gibberellin. Labeled proteins from soluble and particulate fractions were analyzed by two-dimensional gel electrophoresis and specific changes in the patterns of protein synthesis were observed upon treatment with gibberellin. Polyadenylated mRNAs from etiolated or green maize shoots and green pea epicotyls treated or not with gibberellin (a 0.5 to 16 hour time course) were assayed by translation in a rabbit reticulocyte extract and separation of products by two-dimensional gel electrophoresis. Both increases and decreases in the levels of specific polypeptides were seen for pea and corn, and these changes were observed within 30 minutes of treatment with gibberellin. Together, these data indicate that gibberellin induces changes in the expression of a subset of gene products within elongating dwarfs. This may be due to changes in transcription rate, mRNA stability, or increased efficiency of translation of certain mRNAs.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Evidence for "gibberellin-like" Substances from Flowering Plants.

Gibberellins are compounds that produce a wide variety of growth responses in flowering plants. Among these responses are stimulation of shoot growth for many plant species,' root elongation in maize,2 induction of bolting and flowering in some biennial and long-day plants,3-resumption of normal growth in certain genetically dwarfed genotypes of maize,6 growth stimulation in dwarf strains of pe...

متن کامل

The Arabidopsis GA1 locus encodes the cyclase ent-kaurene synthetase A of gibberellin biosynthesis.

The first committed step in the gibberellin (GA) biosynthetic pathway is the conversion of geranylgeranyl pyrophosphate (GGPP) through copalyl pyrophosphate (CPP) to ent-kaurene catalyzed by ent-kaurene synthetases A and B. The ga1 mutants of Arabidopsis are gibberellin-responsive male-sterile dwarfs. Biochemical studies indicate that biosynthesis of GAs in the ga1 mutants is blocked prior to t...

متن کامل

Effect of Securigera Securidaca Seed on the Gene Expression of Pulmonary Hypertensive Broiler Chickens Induced by Cool Temperature and High-Altitude

Securigera securidaca (Fabaceae) or goat pea is growing wild in West Asia, Europe and Africa as an annual herb. In Persian, it is called “Gandeh Talkheh”. The seeds of goat pea are applied in the traditional medicine to treat different dysfunctions e.g., hyperlipidemia and hypertension. This study was done at altitude of 2100 m above sea level. The effects of dietary Securigera securidaca seed ...

متن کامل

Dynamic changes of hemodynamic parameters and cardiac transcription of sirtuins in adaptive and mal-adaptive phases of pressure overload-induced hypertrophy in rats

Introduction: The aim of the study was to investigate the structural and hemodynamic changes as well as cardiac transcriptional profile of the key regulatory proteins, sirtuins family (SIRT1-7), in adaptive and mal-adaptive phases of left ventricular hypertrophy (LVH). Methods: LVH was induced in male Wistar rats (190±20g) by abdominal aortic banding. The third and sixteenth weeks post-surgery ...

متن کامل

Cover crop effects on the fate of N in sweet maize (Zea mays L. saccharata Sturt.) production in a semiarid region

This research aimed to determine the effects of different cover crops and application ofbio-fertilizer on dynamic of nitrogen in the soil and sweet maize yield. Also, we evaluated theeffect of fall–winter species (common vetch, field pea, winter oats, fodder kale) and a mixtureof vetch and field pea with oats used as cover crops, as such as dead organic mulch andtraditional variant, without cov...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Plant physiology

دوره 83 1  شماره 

صفحات  -

تاریخ انتشار 1987